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Open Access Article

International Journal of Clinical Research. 2022; 6: (6) ; 44-51 ; DOI: 10.12208/j.ijcr.20220255.

Serum MMP-11 is a potential biomarker for the diagnosis and prognosis of small cell lung cancer
血清MMP-11是小细胞肺癌诊断及评估预后的潜在生物标志物

作者: 孙任成, 吴振, 张玉丽, 牛国宇, 于渊 *

潍坊市中医院肿瘤外科 山东潍坊

潍坊医学院 山东潍坊

潍坊市人民医院肿瘤内科 山东潍坊

*通讯作者: 于渊,单位:潍坊市人民医院肿瘤内科 山东潍坊;

发布时间: 2022-08-15 总浏览量: 665

摘要

目的 探讨血清基质金属蛋白酶11(MMP-11)在诊断小细胞肺癌(SCLC)及评估预后中的应用价值。方法 收集110例SCLC患者和60例健康人的血清,采用酶联免疫吸附法(ELISA)检测两组间MMP-11的差异,并分析血清MMP-11与小细胞肺癌临床病理特征、化疗疗效及总生存时间(OS)的相关性。结果 治疗前SCLC患者血清MMP-11水平(28.2±13.8 ng/ml)明显高于健康对照组(12.1±5.5ng/ml;p < 0.001),ROC曲线分析显示:曲线下面积(AUC)为0.849,95% CI(0.7930-0.9050),p<0.01。当截断值为18.35 ng/ml时,血清MMP-11诊断SCLC的敏感性、特异性、阳性预测值(PPV)和阴性预测值(NPV)分别为73.6%、83.3%、81.9%和76.0%。Kaplan-Meier(K-M)生存分析显示:小细胞肺癌化疗后血清MMP-11较高者(≥25ng/mL)的OS劣于较低者(<25ng/mL)的OS(中位OS:15.0 vs 23.3个月,P =0.024)。多因素Cox回归分析显示,化疗后血清MMP-11水平是OS的独立影响因素。(风险比[HR] = 1.617 [95% CI,1.126-2.648],P =0.026)。结论 血清MMP-11有望成为SCLC诊断及评估预后的一种新的血清肿瘤标志物。

关键词: 小细胞肺癌;基质金属蛋白酶11;血清生物标志物;化疗效果;预后

Abstract

Objective To explore the value of serum matrix metalloproteinase-11 (MMP-11) in the diagnosis and prognosis of small cell lung cancer (SCLC).
Methods The serum of 110 patients with SCLC and 60 healthy people were collected. The difference of MMP-11 between the two groups was detected by enzyme-linked immunosorbent assay (ELISA), and the correlation between serum MMP-11 and clinicopathological features, chemotherapy efficacy, overall survival time (OS) of small cell lung cancer was analyzed.
Results The level of serum MMP-11 in SCLC patients before treatment (28.2 ± 13.8 ng/ml) was significantly higher than that in healthy control group (12.1 ± 5.5 ng/ml; P < 0.001). ROC curve analysis showed that the area under the curve (AUC) was 0.849, 95% CI (0.7930-0.9050), P < 0.01. When the cutoff value was 18.35 ng/ml, the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of serum MMP-11 in the diagnosis of SCLC were 73.6%, 83.3%, 81.9% and 76.0%, respectively. Kaplan Meier (K-M) survival analysis demonstrated that the OS of patients with high serum MMP-11 (≥ 25ng/ml) was worse than that of patients with low serum MMP-11 (< 25ng/ml) (median OS: 15.0 vs 23.3 months, P=0.024). Multivariate Cox regression analysis indicated that the level of serum MMP-11 after chemotherapy was an independent influencing factor of OS. (hazard ratio [HR] = 1.617 [95% CI, 1.126-2.648], P = 0.026).
Conclusion   Serum MMP-11 could become a new serum tumor marker for the diagnosis and prognosis of SCLC in prospect.

Key words: Small cell lung cancer; matrix metalloproteinase-11; serum biomarkers; chemotherapy effect; prognosis

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引用本文

孙任成, 吴振, 张玉丽, 牛国宇, 于渊, 血清MMP-11是小细胞肺癌诊断及评估预后的潜在生物标志物[J]. 国际临床研究杂志, 2022; 6: (6) : 44-51.