Evaluation of testicular damage using UCHL-1 in a rat model of cryptorchidism
隐睾大鼠模型中利用UCHL-1评估睾丸损害相关研究

摘要

目的 探讨小鼠隐睾模型睾丸组织病理变化与血清UCHL-1及相关性激素水平之间的关系。方法 饲养ICR雄性小鼠50只，利用随机数字表将大鼠随机分为三组：即假手术组，单侧隐睾组和双侧隐睾组。单侧组与双侧组采取手术方式固定对应睾丸至腹腔内，假手术组打开腹腔后睾丸组织未做特殊处理。取双侧睾丸组织清洗后称重，甲醛溶液浸泡保存。用ELISA试剂盒酶联免疫法测量血清激素水平，睾丸组织HE染色，光学显微镜下观察依据Johnsen评分标准进行评分。结果 各组实验鼠体重之间差异均无统计学意义(P<0.05)。睾丸重量，睾丸体积：单侧组健侧睾丸与患侧睾丸比较、双侧组平均睾丸体积（重量）与假手术组平均睾丸体积（重量）比较差异均有统计学意义(P<0.05)。血清AMH和INH-B浓度。单侧组、双侧组和假手术组血清相关值比较，三组数据差异均有统计学意义(P<0.05)。结论 隐睾症可引起小鼠睾丸组织功能障碍，可认为血清UCHL-1是一种简便、准确的血清标志物，它可以很好地评估隐睾睾丸组织的功能。

Abstract

Objective: To investigate the relationship between the histopathological changes of testis in a mouse cryptorchidism model and the levels of serum UCHL-1 and related sex hormones.
Methods Fifty ICR male mice were reared, and the rats were randomly divided into three groups using a random number table: sham operation group, unilateral cryptorchidism group and bilateral cryptorchidism group. In the unilateral group and bilateral group, the corresponding testis was surgically fixed into the abdominal cavity, and the testis tissue in the sham operation group was not treated with special treatment after the abdominal cavity was opened. The bilateral testicular tissues were washed, weighed, and immersed in formaldehyde solution for preservation. Serum hormone levels were measured by enzyme-linked immunosorbent assay (ELISA kit), testicular tissue was stained with HE, observed under a light microscope and scored according to the Johnsen scoring standard.
Results There was no significant difference in the body weight of the experimental mice in each group (P<0.05). Testicular weight, testis volume: There were statistically significant differences between the contralateral testis and the affected testis in the unilateral group, and the average testis volume (weight) in the bilateral group and the sham operation group (P<0.05). Serum AMH and INH-B concentrations. Compared with the unilateral group, the bilateral group and the sham operation group, there were statistically significant differences among the three groups (P<0.05).
Conclusion   Cryptorchidism can cause testicular tissue dysfunction in mice, and it can be considered that serum UCHL-1 is a simple and accurate serum marker, which can well evaluate the function of testicular tissue in cryptorchidism.