Application of chemiluminescence immunoassay in tumor biomarker test and its effect on improving diagnostic accuracy
化学发光免疫法在肿瘤生物标志物检验中的应用对提升诊断准确率的作用分析

摘要

目的 分析化学发光免疫法在肿瘤生物标志物检验中的应用对提升诊断准确率的作用。方法 在我院2020年6月至2021年6月收治的肿瘤患者中，择取120例作为观察组；择取同期在我院进行体检的健康者120名作为对照组。以化学发光免疫法来对研究对象进行肿瘤生物标志物检验，通过血清中癌胚抗原（CEA）、糖类抗原199（CA199）、糖类抗原153（CA153）、甲胎蛋白（AFP）、糖类抗原125（CA125）几类指标来对其肿瘤状况进行诊断，并以病理检查结果为参照，对其加以比较。结果 观察组和对照组患者的CEA、CA199、CA153、AFP、CA125比较，差异显著（P＜0.05），且以前者偏高。参照病理检查，化学发光免疫法的诊断准确率为97.50%（117/120），差异无统计学意义（P＞0.05）。结论 以化学发光免疫法来进行肿瘤生物标志物检验，可提高诊断准确率，具有较高的临床推广价值。

Abstract

Objective: To analyze the role of chemiluminescence immunoassay in the detection of tumor biomarkers in improving the diagnostic accuracy.
Methods: 120 tumor patients treated in our hospital from June 2020 to June 2021 were selected as the observation group; 120 healthy persons who underwent physical examination in our hospital in the same period were selected as the control group. The study subjects were tested for tumor biomarkers by chemiluminescence immunoassay. The tumor conditions were diagnosed by serum carcinoembryonic antigen (CEA), carbohydrate antigen 199 (CA199), carbohydrate antigen 153 (CA153), alpha fetoprotein (AFP) and carbohydrate antigen 125 (CA125), and compared with the results of pathological examination.
Results: there were significant differences in CEA, CA199, CA153, AFP and CA125 between the observation group and the control group (P< 0.05). Referring to pathological examination, the diagnostic accuracy of chemiluminescence immunoassay was 97.50% (117 / 120), and the difference was not statistically significant (P> 0.05).
Conclusion  : using chemiluminescence immunoassay to detect tumor biomarkers can identify five tumor biomarkers, improve the diagnostic accuracy, and has high clinical value.