摘要
目的 探讨CDC45及CDK4在肝细胞癌中的表达、临床意义及其对HCC细胞增殖、侵袭及凋亡的影响，为揭示肝细胞癌发病机制提供实验依据。方法 选取2019年6月至2021年5月院收治的肝细胞癌患者20例为研究对象。人肝细胞癌细胞 SK-N-SH购自美国ATCC公司。应用SPSS21.0统计学软件分析数据。结果 与肿瘤旁组织比较，肝细胞癌组织中CDC45的表达水平显著降低（P＜0.05），CDK4的表达水平显著升高（P＜0.05）。相较于miR-NC组，CDC45组SK-N-SH细胞活力显著降低，组间差异有统计学意义（P＜0.05），迁移与侵袭细胞数显著减少（P＜0.05），Cyclin D1、MMP-2蛋白表达水平显著降低，组间差异有统计学意义（P＜0.05），P21、E-cadherin蛋白表达水平显著升高，组间差异有统计学意义（P＜0.05）。Western blot检测结果显示，与miR-NC组比较，CDC45组SK-N-SH细胞中CDK4的表达水平显著降低，组间差异有统计学意义（P＜0.05）；与anti-miR-NC组相比，anti-CDC45组SK-N-SH细胞中CDK4的表达水平显著升高（P＜0.05）。结论 CDC45在肝细胞癌组织中呈低表达，CDK4在肝细胞癌组织中呈高表达，体外细胞实验结果显示抑制CDK4的表达可抑制肝细胞癌细胞增殖、迁移及侵袭，表明沉默CDK4基因表达可能通过抑制MMP-2表达而抑制黑色素瘤细胞增殖及侵袭。
关键词： CDC45；CDK4；肝细胞癌
Abstract
Objective: To investigate the expression and clinical significance of CDC45 and CDK4 in hepatocellular carcinoma and their effects on the proliferation, invasion and apoptosis of HCC cells, and to provide experimental basis for revealing the pathogenesis of hepatocellular carcinoma. Methods Twenty patients with hepatocellular carcinoma who were admitted to our hospital from June 2019 to May 2021 were selected as the research objects. Human hepatocellular carcinoma cells SK-N-SH were purchased from ATCC Company, USA. SPSS 21.0 statistical software was used to analyze the data. Results Compared with the adjacent tissue, the expression level of CDC45 in hepatocellular carcinoma tissue was significantly decreased (P<0.05), and the expression level of CDK4 was significantly increased (P<0.05). Compared with the miR-NC group, the viability of SK-N-SH cells in the CDC45 group was significantly decreased, and the difference between the groups was statistically significant (P<0.05). The expression level of -2 protein was significantly decreased, and the difference between groups was statistically significant (P<0.05). The protein expression levels of P21 and E-cadherin were significantly increased, and the difference between groups was statistically significant (P<0.05). Western blot results showed that compared with the miR-NC group, the expression level of CDK4 in SK-N-SH cells in the CDC45 group was significantly decreased, and the difference between the groups was statistically significant (P<0.05). Compared with the anti-CDC45 group, the expression level of CDK4 in SK-N-SH cells in the anti-CDC45 group was significantly increased (P<0.05). Conclusion The expression of CDC45 is low in hepatocellular carcinoma tissue, and the expression of CDK4 is high in hepatocellular carcinoma tissue. The results of in vitro cell experiments show that inhibiting the expression of CDK4 can inhibit the proliferation, migration and invasion of hepatocellular carcinoma cells, indicating that silencing CDK4 gene expression may It inhibits the proliferation and invasion of melanoma cells by inhibiting the expression of MMP-2.
Key words： CDC45; CDK4; Hepatocellular carcinoma
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