Analysis of anti-inflammatory effects of endophytic bacteria metabolites of licorice in vivo and in vitro
甘草内生菌代谢物的体内外抗炎作用分析

摘要

目的 探究甘草内生菌代谢物的体内外抗炎作用分析。方法 模型组把1 mg•L-1脂多糖(LPS)加入，把巨噬细胞 RAW264.7接种于96 孔板中培养，各受试药物组分别把10，20，40 mg•L-1内生菌发酵物或甘草水提液浸膏加入其中，2h时间之后再把 LPS1 mg•L-1加入培养，实现对IL-1β、PGE2、 NO含量的检测。共72只Wistar 大鼠，随机划分成观察组（内生菌代谢物组）及对照组（阳性对照组）各36例，连续灌胃给药5 d。比较分析对及分泌炎症因子和巨噬细胞 RAW264.7 存活率的影响，大鼠外周血白细胞计数、炎症相关因子的含量等。结果 相较于对照组，观察组的大鼠外周血白细胞数量较高，且JTZB018、JTZB059、JTZB006、JTZB060、JTZB063均高于对照组，显示存在统计学意义（P＜0.05）；对照组IL-6、TNF-α均低于观察组，IL-10较高，且JTZB018、JTZB059、JTZB006、JTZB060、JTZB063的IL-6、TNF-α均高于对照组，显示存在统计学意义（P＜0.05）。结论 针对 LPS 诱导的炎症模型，(JTZB006、JTZB060、JTZB063）3株甘草内生菌代谢物，有体外及体内抗炎作用，安全性较高，具有临床应用价值。

Abstract

Objective To explore the anti-inflammatory effects of endophytic bacteria metabolites in licorice in vivo and in vitro.
Methods: 1 mg•L-1 lipopolysaccharide (LPS) was added to the model group, and macrophages RAW264.7 were inoculated in 96-well plates for culture. Each test drug group was treated with 10, 20, 40 mg•L-1 The fermentation product of endophytic bacteria or the aqueous extract of licorice was added, and LPS1 mg•L-1 was added to the culture after 2h to achieve the detection of IL-1β, PGE2, and NO content. A total of 72 Wistar rats were randomly divided into an observation group (endophytic metabolite group) and a control group (positive control group) with 36 cases each, and they were given continuous intragastric administration for 5 days. Comparative analysis of the effects on the secretion of inflammatory factors and the survival rate of macrophages RAW264.7, the count of white blood cells in the peripheral blood of rats, and the content of inflammation-related factors.
Results: Compared with the control group, the number of peripheral blood leukocytes in the observation group was higher, and JTZB018, JTZB059, JTZB006, JTZB060, and JTZB063 were all higher than those in the control group, showing statistical significance (P＜0.05); the control group IL -6, TNF-α are lower than the observation group, IL-10 is higher, and the IL-6 and TNF-α of JTZB018, JTZB059, JTZB006, JTZB060, JTZB063 are higher than those of the control group, showing statistical significance (P< 0.05).
Conclusion  : For the LPS-induced inflammation model, (JTZB006, JTZB060, JTZB063) 3 strains of licorice endophytic metabolites have anti-inflammatory effects in vitro and in vivo, with high safety and clinical application value.