摘要
目的 观察表皮生长因子受体单克隆抗体mAb225与放射对体外培养的腺样囊性癌细胞凋亡的影响。方法 应用CCK8实验方法分析不同浓度的mAb225对腺样囊性癌细胞凋亡的影响，应用流式细胞仪分析单独应用表皮生长因子受体单克隆抗体mAb225、放射以及两者联合应用对腺样囊性癌细胞凋亡的影响，并观察其量效关系。结果 加入一定浓度的mAb225作用一段时间后细胞的增殖出现明显的抑制，并且随着加入mAb225浓度的增大，对肿瘤细胞增殖的抑制作用越明显。1μg/mL mAb225组和6Gy放射组的腺样囊性癌细胞凋亡率是腺样囊性癌细胞自然凋亡率的1～2倍，两者联合组的腺样囊性癌细胞凋亡率可以提高6～7倍。结论 mAb225可以明显提高腺样囊性癌细胞的放射后的细胞凋亡率。
关键词： 单克隆抗体mAb225；放射；凋亡
Abstract
Objective To observe the effect of mab225 and Radiation on the Apoptosis of cultured Adenoid Cystic Carcinoma cell. Methods CCK8 was used to analyze the effect of different concentrations of mAb225 on apoptosis of adenoid cystic cancer cells. Flow cytometry was used to analyze the effect of egfr monoclonal antibody mAb225, radiation and combination of both on apoptosis of adenoid cystic cancer cells, and the dose-effect relationship was observed. Results after a certain concentration of Mab225 was added for a period of time, the proliferation of cells was significantly inhibited. The apoptosis rate of adenoid cystic cancer cells in 1μg/mL mAb225 group and 6Gy group was 1 to 2 times that of natural apoptosis rate of adenoid cystic cancer cells, and the combination of the two could increase 6 to 7 times. Conclusion mAb225 can significantly improve the apoptosis of tumor cells after radiation.
Key words： Mab225; Radiation; Apoptosis
参考文献 References
[1] 王成刚,牛雅琪,马亮,等.口腔颌面部两种恶性肿瘤细胞系的外泌体对比研究[J].临床口腔医学杂志,2019,35(10): 583-586.
[2] 雷印涛,李俊福,姜娟,等.PUMA在唾液腺腺样囊性癌中的表达及临床意义[J].上海口腔医学,2016,25(03):314-316.
[3] 米静,张明宾,万光勇.槲皮素对人腺样囊性癌ACC-M细胞增殖和凋亡的影响及机制研究[J].中国口腔颌面外科杂志,2017,15(02):121-126.
[4] 王宇琛,王辰,刘娜,等.人脱落乳牙牙髓干细胞促进破骨细胞形成的体外实验研究[J].临床口腔医学杂志,2018, 34(04):195-199.
[5] 杨鹏,滕红丽,罗雪兰,等.miR-24对肝癌MHCC97H细胞增殖、凋亡及迁移能力的影响[J].广西医科大学学报, 2019,36(09):1397-1402.
[6] 李发凯,张芳,陆远,等.紫草素促进肺癌A549细胞凋亡[J].现代生物医学进展,2018,18(09):1611-1615+1673.
[7] 赵丽,华夏,谭晔.内质网相关降解蛋白Derlin-1通过抑制ERK信号通路抗人结肠癌细胞增殖[J].中国临床药理学与治疗学,2018,23(12):1353-1358.
[8] 潘春玲,吕雪雯,王宏岩,等.牙龈卟啉单胞菌内化牙周膜干细胞抑制成骨分化的能力[J].中国医科大学学报,2019, 48(08):678-682.
[9] 高轶男,马艳萍.microRNA-28-5p在多发性骨髓瘤的表达分析与表观遗传学研究[J].中国实验血液学杂志,2019, 27(05):1540-1547.
[10] 吕杰,韩婵琳,何文,等.卵巢早衰患者来源特异干细胞分化为单倍体细胞的研究[J].新医学,2019,50(04):249-256.
[11] 朱礼阳,许春伟,于忠和.埃克替尼治疗37例晚期非小细胞肺癌疗效分析[J].临床与病理杂志,2016,36(09):1340-1344.
[12] Anshi Jain , Devi Charan Shetty , Ajit Singh Rathore, et al. Characterization and localization of c-kit and epidermal growth factor receptor in different patterns of adenoid cystic carcinoma[J].Journal of Cancer Research and Therapeutics, 2016, 12 (2)：834-839。
[13] 林海峰,曲杨,张海青,等.肺原发性腺样囊性癌的临床病理特征及PD-1、PD-L1、EGFR、ALK基因检测的临床意义[J].诊断病理学杂志,2019,26(04):219-223.