摘要
口腔鳞癌（OSCC）进展受肿瘤微环境中CAFs调控，但其通过sEVs影响OSCC的机制不明。本研究发现CAFs高表达IGF-1（较正常成纤维细胞高3.2倍），其sEVs可被OSCC细胞摄取，促进增殖（1.8倍）和克隆形成（2.3倍）。机制上，CAFs-sEVs通过IGF-1激活AKT/ERK通路，敲减IGF-1使促增殖效应降低65%。临床证实CAFs密度与不良预后相关。该研究首次揭示CAFs通过sEVs-IGF-1轴驱动OSCC进展，为靶向治疗提供新靶点。
关键词： 癌相关成纤维细胞；胰岛素样生长因子；口腔鳞状细胞癌
Abstract
The progression of oral squamous cell carcinoma (OSCC) is regulated by CAFs in the tumor microenvironment, but the mechanism by which it affects OSCC through sEVs is unclear. This study found that CAFs highly expressed IGF-1 (3.2 times higher than normal fibroblasts), and their sEVs could be taken up by OSCC cells, promoting proliferation (1.8 times) and clone formation (2.3 times). Mechanistically, CAFs-sEVs activate the AKT/ERK pathway through IGF-1, and knockdown of IGF-1 reduces the proliferative effect by 65%. Clinically confirmed, the density of CAFs is associated with a poor prognosis. This study reveals for the first time that CAFs drive the progression of OSCC through the sEVs-IGF-1 axis, providing a new target for targeted therapy.
Key words： Cancer-associated fibroblasts; Insulin-like growth factor-1; Oral squamous cell carcinoma
参考文献 References
[1] 盛嘉昊.癌相关成纤维细胞源性细胞外囊泡携带IGF-1促进口腔鳞状细胞癌细胞增殖研究[D].大连医科大学,2021.
[2] 耿华,李磊,杨杰,等.过氧化物还原酶-4在口腔鳞状细胞癌中的表达及对癌细胞增殖、迁移和侵袭的影响[J].口腔疾病防治,2025,33(04):278-288.
[3] 李曦雯,王瑾,朱瑶琪,等.lncRNA RARB-AS2对口腔鳞状细胞癌细胞增殖、细胞周期和侵袭的影响及分子机制[J].山西医科大学学报,2023,54(08):1038-1044.
[4] 王倩,彭晖,章礼玉,等.口腔鳞状细胞癌患者皮瓣修复术后肺部感染多重耐药菌危险因素分析[J/OL].口腔疾病防治,1-12[2025-05-22].
http://kns.cnki.net/kcms/detail/44.1724.r.20250521.1756.002.html.
[5] 赵惠婷,韩伟,罗观发,等.基于空间代谢组学揭示口腔鳞状细胞癌诊断标志物[J].口腔医学研究,2025,41(04):287-292.
[6] 徐小兵,张红英,范新德.血红蛋白与红细胞分布宽度比值对口腔鳞状细胞癌化疗敏感性及预后的预测价值[J].国际检验医学杂志,2025,46(09):1082-1086.
[7] 秦欢,王洁,杨杰,等.Polo样激酶4调控口腔鳞状细胞癌侵袭、增殖和凋亡的分子机制研究[J].中国癌症杂志,2025, 35(04):365-375.
[8] 赵惠婷,韩伟,罗观发,等.基于空间代谢组学揭示口腔鳞状细胞癌诊断标志物[J].口腔医学研究,2025,41(04):287-292.