Preparation of hydrogel dressing loaded with miR-27b inhibitor and its basic research in diabetes foot
负载miR-27b抑制剂水凝胶敷料的制备及在糖尿病足中的基础研究

摘要

目的 分析在糖尿病足中制备和应用负载miR-27b抑制剂水凝胶敷料对糖尿病足的作用。方法 将2021年1月-2023年12月作为研究开展阶段，选择糖尿病足大鼠30只，所有试验对象每天腹腔均注射55 mg/kg链脲佐菌素，持续一周，建立糖尿病足溃疡的模型，在糖尿病大鼠的后足上做4×4 mm的伤口，再将30例糖尿病足大鼠分3组，空白对照组、高糖不含miR-27b 抑制剂水凝胶组（简称：不含miR-27b组）、高糖含miR-27b 抑制剂水凝胶组（简称：富含miR-27b组），每组各10只。于治疗后的第 2 周、4 周及 8 周于相应创面上取材，观察三组大鼠炎症相关因子IL-1β、TNF-α、IL-10、PMNs在各组细胞中的含量以及创面肉芽组织生长情况和PI3K/AKT通路相关蛋白在各组细胞中的表达。结果 富含miR-27b组2周、4周、8周炎症相关因子IL-1β、TNF-α、IL-10在各组细胞中的含量低于空白对照组以及不含miR-27b组, 肉芽组织生长率，以及PI3K/AKT通路相关蛋白在各组细胞中的表达优于空白对照组以及不含miR-27b组，差异对比有统计学意义，（P<0.05）。结论 负载miR-27b抑制剂水凝胶敷料应用糖尿病足创面可降低炎性因子，调控PI3K/AKT通路相关蛋白，促进创面肉芽组织成熟，促使由结缔组织转变为瘢痕组织。

Abstract

Objective To analyze the effect of the preparation and application of hydrogel dressing loaded with miR-27b inhibitor on diabetic foot.
Methods The study was carried out from January 2021 to December 2023. Thirty diabetic foot rats were selected. All experimental subjects were intraperitoneally injected with 55 mg/kg streptozotocin every day for one week to establish a model of diabetic foot ulcer. A 4×4 mm wound was made on the hind feet of diabetic rats. The 30 diabetic foot rats were divided into three groups: blank control group, high-glucose hydrogel group without miR-27b inhibitor (abbreviated as: no miR-27b group), and high-glucose hydrogel group with miR-27b inhibitor (abbreviated as: rich in miR-27b group), with 10 rats in each group. At the 2nd, 4th and 8th week after treatment, samples were collected from the corresponding wounds to observe the contents of inflammatory factors IL-1β, TNF-α, IL-10 and PMNs in the cells of each group, the growth of granulation tissue in the wound and the expression of PI3K/AKT pathway-related proteins in the cells of each group.
Results The contents of inflammatory factors IL-1β, TNF-α and IL-10 in the cells of the miR-27b-rich group at 2, 4 and 8 weeks were lower than those in the blank control group and the group without miR-27b, and the granulation tissue growth rate and the expression of PI3K/AKT pathway-related proteins in the cells of each group were better than those in the blank control group and the group without miR-27b, and the differences were statistically significant (P<0.05).
Conclusion   The application of hydrogel dressing loaded with miR-27b inhibitor on diabetic foot wounds can reduce inflammatory factors, regulate PI3K/AKT pathway-related proteins, promote the maturation of granulation tissue in the wound, and promote the transformation from connective tissue to scar tissue.