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Open Access Article

International Journal of Clinical Research. 2024; 8: (8) ; 174-180 ; DOI: 10.12208/j.ijcr.20240333.

Detection and analysis of serum bile acid profiles using liquied chromatography tandem mass spectrometry in patients with inflammatory bowel disease
炎症性肠病患者血清胆汁酸谱的液质检测与结果分析

作者: 刘楚 *, 董维, 张吉, 沈凌晓, 吴卫甲

杭州凯莱谱医学检验实验室有限公司 浙江杭州

*通讯作者: 刘楚,单位:杭州凯莱谱医学检验实验室有限公司 浙江杭州;

发布时间: 2024-08-23 总浏览量: 65

摘要

目的 通过液相色谱串联质谱法(LC-MS/MS)方法准确检测炎症性肠病患者血清中的胆汁酸谱水平,探讨血清胆汁酸谱的检测结果在炎症性肠病患者的临床诊断、早期预防及治疗靶点中的应用价值。方法 入组2019年9月-2023年11月间来我司进行血清胆汁酸谱检测的412例克罗恩病患者、198例溃疡性结肠炎患者、121例健康体检者,采用LC-MS/MS检测其血清胆汁酸中的15种亚组份,包括:胆酸(CA)、甘氨胆酸(GCA)、牛磺胆酸(TCA)、鹅脱氧胆酸(CDCA)、甘氨鹅脱氧胆酸(GCDCA)、牛磺鹅脱氧胆酸(TCDCA)、脱氧胆酸(DCA)、甘氨脱氧胆酸(GDCA)、牛磺脱氧胆酸(TDCA)、石胆酸(LCA)、甘氨石胆酸(GLCA)、牛磺石胆酸(TLCA)、熊脱氧胆酸(UDCA)、甘氨熊脱氧胆酸(GUDCA)、牛磺熊脱氧胆酸(TUDCA),并进行各组之间结果比较。结果 与健康组相比较,溃疡性结肠炎组和克罗恩病组的初级胆汁酸水平要高于健康组,其中游离型的CA和CDCA要明显高于健康组,差异均有统计学意义(P<0.05);次级胆汁酸大部分要低于健康组,其中DCA、LCA、GDCA、GLCA的差别均有统计学意义(P<0.05)。结论 血清胆汁酸谱的LC-MS检测结果可能作为炎症性肠病临床诊断及早期预防、治疗的重要辅助手段。

关键词: 炎症性肠病;血清胆汁酸谱;液相色谱串联质谱;肠道菌群

Abstract

Objective: To explore the change of serum bile acid concentrations using liquid chromatography tandem mass spectrometry (LC-MS/MS) and assess the application of the results in the clinical diagnosis, early prevention and treatment of inflammatory bowel disease.
Methods The relevant clinical data was collected from 412 Crohn disease patients 、198 ulcerative colitis patients and 121healthy individuals undergoing serum bile acid testing from September 2019 - November 2023 at Hangzhou CALIBRA Medical Laboratory . Fifteen serum bile acids were detected by liquid chromatography tandem mass spectrometry.
Results Compared with the healthy control group, the primary bile acids in the ulcerative colitis group and the Crohn's disease group were higher, such as the changed CA and CDCA levels which were statistically significant (P<0.05). The secondary bile acids were mostly lower in the ulcerative colitis group and the Crohn's disease group, such as the changed DCA, LCA, GDCA, and GLCA levels which were statistically significant (P<0.05).
Conclusion   Serum bile acid profiling using LC-MS/MS could possibly be used as an important supplement for the clinical diagnosis, early prevention and treatment of inflammatory bowel disease.

Key words: Inflammatory bowel disease; Serum bile acid profile; Liquid chromatography tandem mass spectrometry; Gut microbiota

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引用本文

刘楚, 董维, 张吉, 沈凌晓, 吴卫甲, 炎症性肠病患者血清胆汁酸谱的液质检测与结果分析[J]. 国际临床研究杂志, 2024; 8: (8) : 174-180.