Expression of telomere/telomerase in ligament tissue of pelvic prolapse organs
端粒/端粒酶在盆腔脱垂器官韧带组织中的表达研究

摘要

目的 研究端粒/端粒酶在盆底结缔组织中的表达，探讨端粒/端粒酶在基因水平参与盆腔器官脱垂（POP）的发生机制。方法 随机选取35-75岁的患者入组40例，实验组为因子宫脱垂行子宫切除术的患者；对照组为因良性疾病行全子宫除的患者。对手术标本的子宫骶韧带、圆韧带组织通过Masson染色、IHC等实验方法检测其中组织细胞的端粒酶、I型胶原（COLL I）、Ⅲ型胶原（COLL III）等指标的表达情况。结果 实验组与对照组COLL I、COLLⅢ的表达有统计学差异，但两组间TERT表达无统计学差异。结论 盆腔器官脱垂患者的子宫韧带中组织COLLⅠ、COLLⅢ表达显著下降，但端粒/端粒酶在盆腔脏器脱垂形成中的影响需进一步的实验验证。

Abstract

Objective To investigate the expression of telomere/telomerase in pelvic connective tissue and explore the mechanism of telomere/telomerase involvement in the occurrence of pelvic organ prolapse (POP) at the gene level.
Methods 40 patients aged 35 to 75 were randomly selected. The experimental group consisted of patients who underwent hysterectomy due to uterine prolapse; The control group consisted of patients who underwent total hysterectomy due to benign uterine diseases. The expression of telomerase, COLI, COLIII and other indicators in the tissue cells of the uterine sacral ligament and round ligament tissues of surgical specimens was detected using experimental methods such as Masson staining and IHC.
Results There was a statistical difference in the expression of COLL I and COLL III between the two groups, but there was no significant difference in TERT expression.
Conclusion   The expression of COLLI and COLLIII in the uterine ligaments of patients with pelvic organ prolapse has decreased significantly, but the effect of telomeres in the formation of pelvic organ prolapse needs to be further experimentally verified.