To explore the influence of insufficient paraffin removal in HE staining on clinical and pathological diagnosis
探讨HE染色中切片脱蜡不足对临床病理诊断的影响

摘要

目的 分析探讨常规石蜡制片中HE染色脱蜡不足对临床病理诊断的影响。方法 随机选取我科2例石蜡组织标本，各切2张白片，每例各取1张白片分别作为对照组和实验组，对照组的切片充分脱蜡后按常规手工HE染色流程制片，实验组的切片快速置于二甲苯后立马取出，并与对照组一同进入后续的染色流程，制片完成后在镜下观察两组切片染色效果并分析。结果 对照组切片染色均匀，组织和细胞结构清楚，有助于病理诊断，实验组切片染色不均匀，部分组织和细胞模糊不清或者无法显色，严重影响病理诊断。结论 脱蜡作为HE染色法的第一个步骤，是染好一张石蜡切片的前提，脱蜡是否彻底决定了染色效果，若切片脱蜡不干净，染料和试剂都难以进入组织细胞，导致染色效果差不利于临床病理诊断，容易造成误诊或漏诊。

Abstract

Objetive To analyze and explore the impact of insufficient HE staining and dewaxing on clinical and pathological diagnosis in routine paraffin sections.
Methods: Randomly select 2 paraffin tissue samples from our department, and cut 2 white slices each. Take 1 white slice from each case as the control group and the experimental group, respectively. The slices of the control group were fully dewaxed and then prepared according to the conventional manual HE staining process. The slices of the experimental group were quickly placed in xylene and immediately removed, and entered the subsequent staining process together with the control group. After the preparation was completed, the staining effects of the two groups of slices were observed under the microscope and analyzed.
Results: In the control group, sections were evenly stained with clear tissue and cell structures, which was helpful for pathological diagnosis. In the experimental group, sections were unevenly stained, and some tissues and cells were blurred or unable to show color, which seriously affected pathological diagnosis.
Conclusion  : As the first step of HE staining, dewaxing is the prerequisite for dyeing a paraffin section. Whether the paraffin section is thoroughly dewaxed determines the dyeing effect. If the section is not dewaxed cleanly, it is difficult for dyes and reagents to enter the tissue cells, resulting in poor dyeing effect, which is detrimental to clinical and pathological diagnosis, and is prone to misdiagnosis or missed diagnosis.