摘要
目的 探讨FDI-6调控叉头框转录因子 M1( FoxM1) 对人肝癌细胞增殖、侵袭的影响。方法 应用定量聚合酶链反应检测肝癌细胞HepG2中加入FDI-6后FoxM1的表达影响，细胞增殖实验和细胞迁移实验检测FoxM1下调空肝癌细胞的增殖和侵袭能力。结果 在HepG2 细胞中，FDI-6≥20μmol/L可明显下调FoxM1的表达。表达。结论 FDI-6能下调 FoxM1的表达，抑制肝癌细胞增殖，导致细胞周期发生停滞，降低细胞迁移能力。
关键词： 肝癌细胞；FDI-6；FoxM1；细胞增殖
Abstract
Objective: To investigate the effects of FDI-6 regulating forkhead box transcription factor M1 (FoxM1) on the proliferation and invasion of human hepatoma cells. Methods: Quantitative polymerase chain reaction was used to detect the expression of FoxM1 in HepG2 cells after adding FDI-6. Cell proliferation and migration tests were used to detect the ability of FoxM1 to down regulate the proliferation and invasion of empty hepatoma cells. Results: In HepG2 cells, FDI-6 ≥ 20 μ Mol/L could significantly down regulate the expression of FoxM1. expression. Conclusion : FDI-6 can down regulate the expression of FoxM1, inhibit the proliferation of hepatoma cells, lead to cell cycle arrest, and reduce the ability of cell migration.
Key words： Hepatoma cells; FDI-6; FoxM1; cell proliferation
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